NFFA project sucessfuly finished

In the NFFA-410 project we applied atomic force and scanning electron microscopy and Raman spectroscopy to analyse brain-derived extracellular vesicles (EVs) from patients with traumatic brain injury. The morphology and composition of these clinical EVs are still poorly characterized in literature due to their

limited availability. But, methods for EV characterization have not yet been standardized in general and particularly not the cutting-edge nanotechnology analyses. During this project we analysed clinical cerebrospinal fluids in which we previously detected changes in EV properties by standard methods including transmission electron microscopy and indirect approaches including detection of EV protein markers and nanoparticles (nanoparticle tracking, western blot and ELISA analyses; Kuharic et al., 2018). NFFA – ABOUT