NanoBioLab – Virtual lab

Extracellular vesicles

NanoBioLab is a virtual lab that gathers scientists focusing on research of extracellular vesicles (EVs)
Our expertises in EV research:
scanning and transmission electron microscopy and atomic force microscopy to describe EV morphology
– immunoblot, ELISA and mass spectrometry to detect EV protein markers
– size-exclusion chromatography to separate EVs from soluble proteins
Our ongoing EV research:
Topic I: Intracranial EVs in severe traumatic brain injury
We have found that EVs from intracranial cerebrospinal fluid from patients with severe traumatic brain injury  increase their size and change protein composition (Kuharic et al. 2018). Our goal is to detect if these induced EVs participate in neuroregeneration.
Topic II- EVs as biomarkers for oocyte maturity
Oocyte maturity is one of the crucial factors of in vitro fertilisation. Our hypothesis is that follicular fluid, which participates in oocyte maturation, might contain EV-related information about oocyte maturity. We plan to characterise EVs from human follicular fluid and assess correlation between oocyte maturity and follicular EVs.